Iquitos is a city of 370,000 in the middle of Amazon jungles of Peru without a road connection with the outside world. Found it while investigating routing failures in OSRM.

Here’s a great travelogue of this fascinating place while trying to research possible car ferry connections. There are none.
Maps lead you to interesting places :)
Discussion
Comment from JJIglesias on 15 April 2016 at 03:12
In the middle of nowhere, just by boat or plane. Big portions of the north of Bolivia are similar in the rain season, no access possible by road, just plane or boat.
Nice Cach